Cytotoxicity in fibroblasts from young and elderly donors from two mouthwashes used to prevent the spread of SARS-CoV-2

In cases of injuries in the oral cavity, mouthwash comes contact with underlying gingival connective tissue and should have its cytotoxicity assessed. However, there is no available evidence if cells elderly donors react differently during vitro assessments mouthwashes. This study aimed to compare evaluation two different types when assessed primary fibroblasts from either young older donors. Primary were collected patients (mean age 66.5 years old) 27.5 old). The cell culture was produced fragments exposed for 24h Perioxidin® Oral B®. A control group unconditioned media, representing 100% survival (negative control), 200mg/mL solution latex used as a positive due well-known toxicity. Both products presented similar dose-dependent cytotoxicity. toxic range, 0.035% 0.00035% 0.06 0.0006% calculated IC50 values very similar, exception B® tested cells, which slightly higher concentration (0.0523 mM). statistical analysis shows significant difference between tests our (p >0.05). These mouthwashes be sparingly prevent spread SARS-CoV-2. use age-matched may not necessary predict differences biological response these products.